Faculty of Engineering, LTH

Denna sida på svenska This page in English

Literature references 2000-2004


  1. Wingren C, Montelius L and Borrebaeck CAK (2004) Mega-dense nanoarrays - the challenge of novel antibody array formats. In: Protein Microarrays (Eds. Schena M and Weaver S). Jones and Bartlett Publ., Sudbury, MA. pp. 339-352.
  2. Fransson J, Ek S, Ellmark P,  Söderlind E,  Borrebaeck CAK and Furebring C (2004) Profiling of internalizing tumor-associated antigens on breast and pancreatic cancer cells by reverse genomics. Cancer Lett 208, 235-242
  3. Barrios Y, Jirholt P and Ohlin M (2004) Length of the antibody heavy chain complementarity determining region 3 as a specificity-determining factor. J Mol Recognit 17, 332-338. (Abstract)
  4. Rudolph MG, Wingren C, Crowley MP, Chien YH and Wilson IA (2004) Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the gammadelta T-cell ligand T10. Acta Crystallogr D Biol Crystallogr 60, 656-664.
  5. Cicortas Gunnarsson L, Nordberg Karlsson E, Albrekt A-S, Andersson M, Holst O and Ohlin M (2004) A carbohydrate binding module as a diversity-carrying scaffold. Protein Eng Des Sel 17, 213-221. (Abstract)
  6. Ellmark P, Ohlin M, Borrebaeck CAK and Furebring C (2004) A novel mammalian display system for selection of protein-protein interactions by receptor engagement. J Mol Recognit 17, 316-322. (Abstract)
  7. Wingren, C and Borrebaeck, CAK (2004) Antibody microarrays. In Encyclopedia of life science. London: Nature Publishing Group.
  8. Ek S, Björck E, Porwit-MacDonald A, Nordenskjöld M and Borrebaeck CAK (2004) Increased expression of Ki-67 in mantle cell lymphoma is associated with de-regulation of several cell cycle regulatory components, as identified by global gene expression analysis. Haematologica 89, 686-695.
  9. Thiel A, Staaf J, Albrekt A-S, Lindstedt M and Schmidt P (2004) Flexible geniom one microarrays im vergleich mit etablierten technologien. BIOspektrum 2004, 444-445.
  10. Wingren C and Borrebaeck CAK (2004) High-throughput proteomics using antibody microarrays. Expert Rev Proteomics 1, 355-364.
  11. Schiött Å, Lindstedt M, Johansson-Lindbom B, Roggen E and Borrebaeck CAK (2004) The CD27-CD4+ memory T cells define a differentiated memory population both at the functional and transcriptional level. Immunology 113, 363-370.
  12. Karlsson AL, Alm R, Ekstrand B, Fjelkner-Modig S, Schiött A, Bengtsson U, Björk L, Hjernø K, Roepstorff P, Emanuelsson CS (2004) Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens. Allergy 59, 1277-1284.
  13. Levander,  F.,  Rögnvaldsson, T.,  Samuelsson, J. and James, P. (2004)   Automated methods for improved protein identification by peptide mass fingerprinting. Proteomics 4, 2594-601.
  14. Palmfeldt,  J.,  Levander, F.,  Hahn-Hägerdal, B.,  and James, P.  (2004)  Acidic proteome of growing and resting Lactococcus lactis metabolizing maltose. Proteomics, 4, 3881-98.
  15. Nakamura, K.,  Aebersold, R.,  Bairoch, A., Dunn, M.,  Celis, J.,  Hanash, S.,  Hochstrasser, D.,  Humphrey-Smith, I.,  James, P.,  Klose, J.,  LaBaer, J.,  Langen, H.,  Mann, M.,  Parekh, R.,  Patterson, S.,  Pearce, C.,  Roepstorff, P.,  Simpson, R.J.,  Tomlinson, I.,  Tsugita, A.,  Yates, J. (2004) From genome to proteome--aim of human proteomic. Seikagaku 76, 1271-1274.
  16. Samuelsson J, Dalevi D, Levander F, Rögnvaldsson T (2004) Modular, scriptable and automated analysis tools for high-throughput peptide mass fingerprinting. Bioinformatics 20, 3628-3635.


  1. Lantto J, Fletcher J and Ohlin M (2003) Binding characteristics determine the neutralizing potential of antibody fragments specific for antigenic domain 2 on glycoprotein B of human cytomegalovirus. Virology  305, 201-209. (Abstract)
  2. Högerkorp C-M, Bilke S, Breslin T, Ingvarsson S, and Borrebaeck CAK (2003) CD44-stimulated human B cells express transcripts specifically involved in immunomodulation and inflammation as analyzed by DNA microarrays. Blood 101, 2307-2313.
  3. Lindstedt M, Johansson Lindbom B and Borrebaeck CAK (2003) Expression of 4-1BB (CD137) on human follicular dendritic cells. Scand J Immunol 57, 305-310. (journal cover)
  4. Gicklhorn D, Eickmann M, Meyer G, Ohlin M and Radsak K(2003) Differential effects of glycoprotein B epitope specific antibodies on human cytomegalovirus induced cell-cell fusion. J Gen Virol 84, 1859-1862. (Abstract)
  5. Karlsson F, Nilsson N, Borrebaeck CAK and Malmborg A-C (2003) The mechanisms of bacterial infection by filamentous phages involves molecular interactions between TolA and phage protein 3 domains. J Bacteriol 185, 2628-2634.
  6. Ellmark P, Furebring C and Borrebaeck CAK (2003) Pre-assembly of the extracellular domains of CD40 is not necessary for rescue of naive B-cells from anti-IgM inuced apopotosis. Immunology 108, 452-457.
  7. Wingren C, Ingvarsson J, Lindstedt M and Borrebaeck CAK (2003) Recombinant antibody microarrays - a viable option? Nat Biotechnol 21, 223.
  8. Svedhem S, Pfeiffer I, Larsson C, Wingren C, Borrebaeck CAK and Höök F (2003) Patterns of DNA-labeled and scFv-antibody-carrying lipid vesicles directed by material-specific immobilization of DNA and supported bilayer formation on an Au/SiO2 template. ChemBioChem 4, 339-343.
  9. Malmborg Hager AC, Ellmark P, Borrebaeck CAK and Furebring C (2003) Affinity and epitope profiling of mouse anti-CD40 monoclonal antibodies. Scand J Immunol 57, 517-524.
  10. Wingren C, Edmundson AB and Borrebaeck CAK (2003) Designing proteins to crystallize through b-strand pairing. Prot Eng 16, 255-264.
  11. Ohlin M and Zouali M (2003) The human antibody repertoire to infectious agents: implications for disease pathogenesis. Mol Immunol 40, 1-11. (Abstract)
  12. Breccia J, Krook M, Ohlin M and Hatti-Kaul R (2003) The search for a peptide ligand targeting the lipolytic enzyme cutinase. Enzyme Microb Technol 33, 244-249.
  13. Johansson-Lindbom B, Ingvarsson S and Borrebaeck CAK (2003) Germinal centers regulate human Th2 development. J Immunol 171, 1657-1666.
  14. Bäck, P.,  Någård, F.,  Bolmsjö, G.,  Bengtsson, S.,  and James, P. (2003)   Automating Gel Image Acquisitio. Journal of Proteome Research 2(6):662-4.


  1. Nilsson N, Karlsson F, Rakonjac J and Borrebaeck CAK (2002) Selective infection of E. coli as a function of a specific molecular interaction. J Mol Recognit 15, 27-32.
  2. Åkesson A, Ingvarsson S, Karlsson F, Levy L, Blanca M, Cuerder SA, Smith JA, Coleman JW and Borrebaeck CAK (2002) Characterization of specific IgE response in vitro against protein and drug allergens using atopic and normal donors. Allergy 57, 193-200.
  3. Lantto J and Ohlin M (2002) Uneven distribution of repetitive sequence motifs in immunoglobulin heavy variable genes. J Mol Evol 54, 346-353. (Abstract)
  4. Johansson-Lindbom B and Borrebaeck CAK (2002) Germinal center B cells constitute a predominant physiological source of IL-4: implication for Th2 development in vivo. J Immunol 168, 3165-3172.
  5. Jirholt P, Borrebaeck CAK and Ohlin M (2002) Antibody evolution in vitro from the centre to the periphery - applied to a human antibody fragment recognising the tumour-associated antigen mucin-1. J Mol Biol 318, 407-416. (Abstract)
  6. Lantto J, Lindroth Y and Ohlin M (2002) Non-germline encoded residues are critical for effective antibody recognition of a poorly immunogenic neutralization epitope on glycoprotein B of human cytomegalovirus. Eur J Immunol 32, 1659-1669. (Abstract)
  7. Ellmark P, Esteban O, Furebring C, Malmborg Hager A-C and Ohlin M (2002) Molecular evolution of antibody genes mimicking receptor revision. Mol Immunol 39, 353-360. (Abstract)
  8. Lantto J, Fletcher J and Ohlin M (2002) A divalent antibody format is required for neutralisation of human cytomegalovirus via antigenic domain 2 on glycoprotein B. J Gen Virol 83, 2001-2005. (Abstract)
  9. Ellmark P, Ottosson C, Borrebaeck CAK, Malmborg Hager A-C and Furebring C (2002) Modulation of the CD40-CD40L interaction using human anti-CD40 single chain antibody fragments obtained from the n-CoDeR phage display library. Immunology 106, 456-463.
  10. Ek S, Högerkorp C-M, Dictor M, Ehinger M and Borrebaeck CAK (2002) Mantle cell lymphomas express a distinct genetic signature affecting lymphocyte trafficking and growth regulation as compared to normal human B cells. Cancer Res 62, 4398-4405.
  11. Lindstedt M, Johansson-Lindbom B and Borrebaeck CAK (2002) Global reprogramming of dendritic cells in response to a concerted action of inflammatory mediators. Int Immunol 14, 1203-1213.
  12. Lantto J and Ohlin M (2002) Functional consequences of insertions and deletions in the complementarity determining regions of human antibodies. J Biol Chem 277, 45108-45114. (Abstract)
  13. Borrebaeck CAK and Wingren C (2002) Introduction. Biotechniques , Suppl.: High-Throughput Proteomics: Protein Arrays, 1.
  14. Steinhauer C, Wingren C, Malmborg Hager AC and Borrebaeck CAK (2002) Single framework recombinant antibody fragments designed for protein chip applications. Biotechniques , Suppl.: High-Throughput Proteomics: Protein Arrays, 38-45.
  15. Borrebaeck CAK and Ohlin M (2002) Antibody evolution beyond Nature. Nat Biotechnol 20, 1189-1190. (Abstract)
  16. James P.  (2002)  Chips for proteomics: a new tool or just hype? Biotechniques. 4(10,) 12-23


  1. Jirholt P, Strandberg L, Jansson B, Krambovitis E, Söderlind E, Borrebaeck CAK, Carlsson R, Danielsson L and Ohlin M (2001) A central core structure in an antibody variable domain determines antigen specificity. Prot Eng 14, 67-74. (Abstract)
  2. Malmborg A-C, Nilsson N and Ohlin M (2001) Selection based on antibody kinetic binding properties. In: Antibody Phage Display: Methods and Protocols (Eds. O'Brien P and Aitken R). Humana Press Inc., Totowa, NJ. pp. 245-253.
  3. Lantto J, Jirholt P, Barrios Y and Ohlin M (2001) Chain shuffling to modify the properties of recombinant antibodies. In: Antibody Phage Display: Methods and Protocols (Eds. O'Brien P and Aitken R). Humana Press Inc., Totowa, NJ. pp. 303-316.
  4. Söderlind E, Carlsson R, Borrebaeck CAK and Ohlin M (2001) The immune diversity in a test tube - non immunised antibody libraries and functional variability in defined protein scaffolds. Comb Chem High Throughput Screening 4, 409-416. (Abstract)
  5. Borrebaeck CAK, Ekström S, Malmborg Hager AC, Nilsson J, Laurell T and Marko-Varga G (2001) Protein chips based on recombinant antibody fragments: a highly sensitive approach as detected by mass spectrometry. Biotechniques 30, 1126-1132.
  6. Borrebaeck CAK and Carlsson R (2001) Human therapeutic antibodies. Curr Opinion Pharmacol 1, 404-408.
  7. Brady K, Fitzgerald S, Ingvarsson S, Borrebaeck CAK and Moynagh PN (2001) CD40 employs p38 MAP kinase in IgE isotype switching. Biochem Biophys Res Commun 289, 276-281.
  8. Furebring C, Speckner A, Mach M, Sandlie I, Norderhaug L, Borrebaeck CAK, Turesson H and Ohlin M (2001) Antibody-mediated neutralization of cytomegalovirus: Modulation of efficacy induced through the IgG constant region. Mol Immunol 38, 833-840. (Abstract)
  9. Norrback KF, Hultdin M, Dahlenborg K, Österman P, Carlsson R and Roos G (2001) Telomerase regulation and telomere dynamics in germinal centers. Eur J Haematol 67, 309-317.
  10. Zhou, X.,  Gonnet, G.,  Hallett, M.,  Münchbach, M.,  Folkers, G.,  and James, P.  (2001)  Cell fingerprinting:  An approach to classifying cells according to mass profiles of digests of protein extract. Proteomics 1, 683-690.
  11. James P.  (2001)  Protein expression analysis: from "tip of the iceberg" to a global method. Dis Markers. 17, 235-46. 


  1. Malmborg AC (1999) Phage display method. In: Real-time analysis of biomolecular interactions - applications of BIACORE (English edition). (Eds.: Nagata K, Handa H ) Springer-Verlag, Tokyo. pp. 187-194.
  2. Nilsson N, Ingvarsson S and Borrebaeck CAK (2000) Immature B cells in bone marrow express Fas/FasL Scand J Immunol 51, 279-284.
  3. Johansson B, Ingvarsson S, Björck P and Borrebaeck, CAK (2000) Human interdigitating dendritic cells induce isotype-switching and IL-13 dependent IgM production in CD40 activated naive B cells. J Immunol 164, 1847-1854.
  4. Nilsson N, Malmborg A-C, and Borrebaeck CAK (2000) The phage infection process: a functional role for the distal linker region of bacteriophage protein 3. J Virol 74, 4229-4235.
  5. Ohlin M, Jirholt P, Thorsteinsdottir HB and Borrebaeck CAK (2000) Understanding human immunoglobulin repertoires in vivo and evolving specificities in vitro. In: The Antibodies, volume 6. (Eds. Zanetti M and Capra JD). Harwood Acad. Publ., Amsterdam. pp. 81-104.
  6. Åkesson A, Ingvarsson S, Brady K, Moynagh P and Borrebaeck CAK (2000) Rapid polarisation of Th2 cells during induction of antigen-specific IgE antibodies in vitro. Clin Exp Allergy 30, 1298-1306.
  7. Dahlenborg K, Pound J, Gordon J, Borrebaeck CAK and Carlsson R (2000) Signals sustaining human immunoglobulin V gene hypermutation in isolated germinal center B cells. Immunology 101, 210-217.
  8. Borrebaeck CAK (2000) Antibodies in diagnostics - from immunoassay to protein chips. Immunol Today 21, 379-382.
  9. Söderlind E, Strandberg L, Jirholt P, Kobayashi N, Alexeiva V, Åberg AM, Nilsson A, Jansson B, Ohlin M, Wingren C, Danielsson L, Carlsson R and Borrebaeck CAK (2000) Recombining germline-derived CDR sequences for creating diverse single-framework antibody libraries. Nat Biotechnol 18, 852-856. (Abstract)
  10. Gavilondo J, Larrick JW and Borrebaeck CAK (2000) Human antibody for therapy. The Immunologist 8, 58-65.
  11. Zafiropoulos A, Kandilogiannaki M, Dahlenborg C, Borrebaeck CAK and Krambovitis E (2000) In vitro induction of somatic mutations in human peripheral B lymphocytes. Int J Mol Med 6, 475-484.
  12. Hoving, S.,  Münchbach, M.,  Quadroni, M.,  Staudenmann, W., and James, P. (2000) Multiple N-terminal tag generation from unseparated protein digests using a novel thioester based degradation reactio. Anal. Chem. 72, 1006-1014.
  13. Brunati, A.,  Contri, A.,  Münchbach, M.,  James, P.,  Marin, O.,  and Pinna, L.  (2000) Grp94 (endoplasmin) co-purifies with and is phosphorylated by Golgi apparatus casein kinas. FEBS Letters.  14, 151-155.
  14. Kahnert, A.,  Vermeij, P.,  Wietek, C.,  James, P.,  Leisinger, T.,  Kertesz, M.A. (2000)  The ssu Locus Plays a Key Role in Organosulfur Metabolism in Pseudomonas putida S-31.  J. Bacteriol. 182, 2869-2878.
  15. Brunati, A.,  Bordin, L,  Clari, G.,  James, P., Quadroni, M.,  Baritono, E.,  Pinna, L.,  and Donella-Deana, A.  (2000) Sequential phosphorylation of protein band III by Syk and Lyn tyrosine kinases in intact human erythrocytes.  Identification of primary and secondary phosphorylation site. Blood.  96, 1550-1557.
  16. Santella, L.,  Kyozuka, K.,  Hoving, S.,  Munchbach, M.,  Quadroni, M., Dainese P, Zamparelli C,  James, P. and Carafoli, E.  (2000)   Breakdown of Cytoskeletal Proteins during Meiosis of Starfish Oocytes and Proteolysis Induced by Calpai. Exp. Cell Res.  25, 117-126.
  17. Münchbach, M.,  Quadroni, M.,  Miotto, G., and James, P.  (2000) Quantitation and de novo sequencing of peptides using isotopic N-terminal tagging with a fragmentation-directing highly basic moiet. Anal. Chem.  72, 4047-4057.

Publications 2005-

Publications 1990-1999


Page Manager: