Dendritic cells in immune regulation and hypersensitivity reactions
Research activities
This research group focus both on basal research of dendritic cell (DC) biology and function in hypersensitivity reactions, as well as more applied research with development of novel test systems and protocols for immunotherapy. We are interested in the mechanistic processes that influence DCs during ongoing hypersensitivity reactions and the factors by which DCs skew the immune response toward different effector T cell responses.
Projects include
- Functional assessment of the role of different subpopulations of DCs in immune regulation and allergy
- Development of in vitro test assays for sensitization to replace animal experimentation
- Development of adjuvants and protein-delivery systems in immunotherapy of cancer and allergy using biodegradable nanoparticles
Common methodologies in cellular immunology include flow cytometry, immunofluorescence microscopy, proliferation assays, PCR, ELISA, Luminex, mRNA and protein microarray analysis, and bioinformatics.
Our research in the media
Cellodling kan ersätta djurförsök
Cellprov kan ersätta djurförsök-1. LUM nr 5. 2010
Cellprov kan ersätta djurförsök-2. LUM nr 5. 2010
Allergy Testing. Lund University on YouTube. April 2010
Med Andra Metoder. Nyhetsblad nr 1, 2010. Stiftelsen Forska Utan Djurförsök
Immunförsvarets kamp. Inslag i TV-programmet Vetenskapslandet i Kunskapskanalen. 5 april 2008
Mänskliga celler skall ersätta djurförsök
Selected references
- Lundberg K, Broos S, Greiff L, Borrebaeck CAK, Lindstedt M (2010) Histamine H4 receptor antagonism inhibits allergen-specific T-cell responses mediated by human dendritic cells. Eur J Pharmacol (in press)
- Lundberg K, Greiff L, Borrebaeck CAK, Lindstedt M (2010) FcεRI levels and frequencies of peripheral blood dendritic cell populations in allergic rhinitis. Hum Immunol 71, 931-933. (Abstract)
- Broos S, Lundberg K, Akagi T, Matsusaki M, Akashi M, Borrebaeck CAK and Lindstedt M (2010) Immunomodulatory nanoparticles as adjuvants and allergen-delivery system to human dendritic cells; implications for specific immunotherapy. Vaccine 28, 5075-5085. (Abstract)
- Weltzien H-U, Corsini E, Gibbs S, Lindstedt M, Borrebaeck CAK, Budde P, Schulz-Knappe P, Thierse H-J, Martin S.F and Roggen E.L (2009) Safe cosmetics without animal testing? Contributions of the EU Project Sens-it-iv. J Verbr Lebensm 4, 41. (Abstract)
- Larsson K, Lindstedt M, Lundberg K, Dexlin L, Wingren C, Korsgren M, Greiff L, Borrebaeck CAK (2009) CD4+ T-cells have a key instructive role in educating dendritic cells in allergy. Int Arch Allergy 149, 1-15. (Abstract)
- Lundberg K, Lindstedt M, Larsson K, Dexlin L, Wingren C, Ohlin M, Greiff, L, Borrebaeck CAK (2008) Augmented Phl p 5-specific Th2 response after exposure of Dendritic Cells to allergen in complex with specific IgE compared to IgG1 and IgG4. Clin Immunol 128, 358-65. (Abstract)
- Santegoets S, Gibbs S, Kroeze K, van de Ven R, Scheper RJ, Borrebaeck CAK, de Gruijl TD, Lindstedt M (2008) Transcriptional profiling of human skin-resident Langerhans cells and CD1a+ dermal dendritic cells: differential activation states suggest distinct functions. J Leukoc Biol 84, 143-51. (Abstract)
- Larsson K, Lindstedt M, and Borrebaeck CAK. (2006) Functional and transcriptional profiling of MUTZ-3. A myeloid cell line acting as a model for dendritic cells. Immunology 117, 156-66. (Abstract)
- Lindstedt M, Lundberg K, and Borrebaeck CAK (2005) Gene family clustering identifies functionally associated subsets of human in vivo blood and tonsillar dendritic cells. J Immunol 175, 4839-46.
- Lindstedt M, Schiött Å, Bengtsson A, Larsson K, Korsgren M, Greiff L, and Borrebaeck CAK (2005) Genomic and functional delineation of dendritic cells and memory T cells derived from grass pollen-allergic patients and healthy individuals. Int Immunol 17, 401.
- Lindstedt M, Schiött Å, Johnsen CR, Roggen E, Johansson-Lindbom B, and Borrebaeck CAK (2005) Individuals with occupational allergy to detergent enzymes display a differential transcriptional regulation and cellular immune response. Clin Exp Allergy 35, 199.
- Roggen EL, Lindstedt M, Borrebaeck C, Verheyen GR (2005) Interactions between dendritic cells and epithelial cells in allergic disease. Toxicol Lett 162, 71. (Abstract)
- Matsusaki M, Larsson K, Akagi T, Lindstedt M, Akashi M, Borrebaeck CAK (2005) Nanosphere induced gene expression in human dendritic cells. Nano Lett 5, 2168-73.
- Schiött Å, Lindstedt M, Johansson-Lindbom B, Roggen E, Borrebaeck CAK (2004) The CD27- memory CD4+ T cells defines a differentiated memory population both at functional and transcriptional level. Immunology 113, 363-70.
- Lindstedt M, Johansson-Lindbom B, Borrebaeck CAK (2003) Expression of CD137 (4-1BB) on Human Follicular Dendritic Cells. Scand J Immunol 57, 305-10. (journal cover)
- Lindstedt M, Johansson-Lindbom B, and Borrebaeck CAK (2002) Global reprogramming of dendritic cells in response to a concerted action of inflammatory mediators. Int Immunol 14, 1203.
Contact information:
Assoc. Professor Malin Lindstedt
Dept. of Immunotechnology, Lund University,
BMC D13, S-221 84 Lund, Sweden
telephone: +46-46-222 9256
e-mail
Page Manager: Mats Ohlin
Webmaster: webmaster@lth.se
Last updated: 2011-08-15