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Immunotechnology

Faculty of Engineering, LTH

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Protein microarray techniques

In this one-week full-time practical course, you will learn about state-of-the-art protein microarray technologies in proteomics. The course involves, lectures, exercises, data analysis, as well as practical work.


Protein microarrays represents a novel technology for multiplexed, high-throughput analysis of crude, complex samples, such as blood, tissue, and cell extracts. In these methods, up to thousands of proteins (e.g. antibodies) are printed in an ordered pattern, an array, onto a solid support (less than 1 cm2) and used as probes for the target analytes of interest. In this manner, thousands of analytes can be analysed simultaneously in a single experiment. The array patterns generated are then transformed into proteomic maps, or fingerprints, revealing the detailed composition of the proteome. In the end, these methodologies will have a major impact on biomedicine, biotechnology, and high-throughput proteomic analysis for years to come, and will provide unique opportunities to perform e.g. disease diagnostics, biomarker discovery, as well as a wide range of biotechnical applications.


The course will present the fundamentals behind designing, fabricating, running and analysing protein microarrays and nanoarrays. Theories behind the different steps are discussed and experiments are performed. The principles behind generating numerous probes using combinatorial biology will be presented. A variety of array designs (e.g. antibody-, reversed protein-, and  lysate-microarrays) and formats (e.g. planar-, bead- and well-based arrays) in both the micro- and nano-scale dimensions will be covered. Present and future applications (e.g. protein expression profiling, biomarker discovery, and disease diagnostics) will be discussed. The practical work will be illustrated by producing, running and analysing antibody microarrays.


The course will be given within the framework of the recently initiated program "Postgraduate Courses in the Life Sciences", where a list of the complete range of courses can be found.

 

The course will be given annually, starting autumn 2014.

Format: One week full time course, with a mixture of lectures, group exercises/discussion and practical work.

No. of students: 6 to 8 students will be accepted.

Points: 3 hp

Time: Week 45, 2014

Course responsible: Assoc. Prof Christer Wingren, tel 046-222 4323

Teaching staff: Assoc. prof. Christer Wingren, MSc. Payam Delfani

Schedule: 1 week full time course. (to be included)

Additional information: Laboratory manuals (to be included).

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For more information about this course, please contact Christer Wingren (phone 046-222 4323).

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